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Chapter 37

The Binding Site MININEPHPLUS is a small, manual nephelometer designed for sFLC analysis (Figure 37.3). The calibration curves are stored on magnetic swipe-cards. Once loaded into the instrument's memory, curve validity is confirmed by assaying control samples. Patient samples are analysed individually by semi-automated addition of reagents into cuvettes within the instrument. The assays show good precision and linearity, and include prozone parameters (Figure 37.3). The instrument may find use in laboratories with low workloads (<10 samples per day) or when the cost of a larger instrument cannot be justified.

κ sFLC λ sFLC
Range at 1/20 3.0 - 72.4 mg/L 4.9 - 98.3 mg/L
Sensitivity at 1/20 3.0 mg/L 4.9 mg/L
Assay time 5 min 5 min
Precision: within-run 7.3% at 55.8 mg/L 5.1% at 80.5 mg/L
5.7% at 18.7 mg/L 3.9% at 29.4 mg/L
4.9% at 4.8 mg/L 3.2% at 7.6 mg/L
Precision: between-run 8.9% at 55.8 mg/L 6.7% at 80.5 mg/L
6.8% at 18.7 mg/L 3.8% at 29.4 mg/L
5.0% at 4.8 mg/L 7.4% at 7.6 mg/L

Table 37.3. Freelite assay performance on the Binding Site MININEPHPLUS.

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