The largest study that has compared the performance of N Latex FLC and Freelite assays in AL amyloidosis was published by Palladini et al. . This included 426 patients with newly-diagnosed AL amyloidosis from two specialist centres (Pavia, n=353; and Limoges, n=73). A poor agreement of quantitative results between the two methods was observed but the diagnostic sensitivity of the Freelite (82%) and N-latex (84%) assays was similar, and both improved to 98% in combination with serum and urine immunofixation. Similar results were reported by Mollee et al. .
Mahmood et al.  confirmed that absolute values reported by Freelite and N Latex FLC assays do not always compare well in AL amyloidosis. In this study, the agreement between κ sFLC results at presentation (n=90) was better than that of λ (κ sFLC R2 = 0.91; λ sFLC R2 = 0.52), as discussed above (Section 8.5.3.). κ/λ sFLC ratios were also discordant between the two assays: 10/90 patients were abnormal by Freelite but normal by N Latex, and 11/90 abnormal by N Latex but normal by Freelite. Similar results were reported by Mollee et al. , but the reason for these differences is not understood.